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endothelial basal medium mcdb131  (US Biological Life Sciences)

 
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    US Biological Life Sciences endothelial basal medium mcdb131
    Endothelial Basal Medium Mcdb131, supplied by US Biological Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/endothelial+basal+medium+mcdb131/endothelial+basal+medium+mcdb131/pm39997005-46-25-28
    Average 90 stars, based on 1 article reviews
    endothelial basal medium mcdb131 - by Bioz Stars, 2026-10
    90/100 stars

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    Article Title: NanoBioAnalytical (NBA) Platform to Decipher Extracellular Vesicles Secreted by Microvascular Endothelial Cells Under Benzo[a]pyrene Exposure.
    Article Snippet: .. Human microvascular endothelial cell line (HMEC-1) was sourced from the Center for Disease Control and Prevention, Atlanta, GA, USA; the cells were cultured in endothelial basal medium MCDB131 (US Biological Life Sciences, Ref E3000-01G, Salem, MA, USA) which contained 10% heat deactivated (56 ◦C, 30 min) fetal bovine serum (FBS, Dutscher, Ref 500105A1A, Bernolsheim, France), L-glutamine (Gibco, Ref 25030, 10 mM final, Paisley, Scotland, UK), penicillin/streptomycin (Gibco, Ref 15140-122, 100 unit/mL final), gentamycin (Gibco, Ref 15750-037, 500 μg/mL final), epithelial growth factor (EGF, Sigma, Ref E9644, 10 ng/mL final, Molsheim, France), and hydrocortisone (Up John, Ref 3400932141159, 1 μg/mL final, Paris, France) in 151.9 cm2 petri dishes (Corning, Ref 353025, Borre, France). .. The medium’s pH was adjusted to 7.6 using sodium bicarbonate (Gibco, Ref 25080-060) and replaced every 2–3 days.

    Article Title: NanoBioAnalytical (NBA) Platform to Decipher Extracellular Vesicles Secreted by Microvascular Endothelial Cells Under Benzo[a]pyrene Exposure
    Article Snippet: .. Human microvascular endothelial cell line (HMEC-1) was sourced from the Center for Disease Control and Prevention, Atlanta, GA, USA; the cells were cultured in endothelial basal medium MCDB131 (US Biological Life Sciences, Ref E3000-01G, Salem, MA, USA) which contained 10% heat deactivated (56 °C, 30 min) fetal bovine serum (FBS, Dutscher, Ref 500105A1A, Bernolsheim, France), L-glutamine (Gibco, Ref 25030, 10 mM final, Paisley, Scotland, UK), penicillin/streptomycin (Gibco, Ref 15140-122, 100 unit/mL final), gentamycin (Gibco, Ref 15750-037, 500 μg/mL final), epithelial growth factor (EGF, Sigma, Ref E9644, 10 ng/mL final, Molsheim, France), and hydrocortisone (Up John, Ref 3400932141159, 1 μg/mL final, Paris, France) in 151.9 cm 2 petri dishes (Corning, Ref 353025, Borre, France). .. The medium’s pH was adjusted to 7.6 using sodium bicarbonate (Gibco, Ref 25080-060) and replaced every 2–3 days.

    Cell Culture:

    Article Title: NanoBioAnalytical (NBA) Platform to Decipher Extracellular Vesicles Secreted by Microvascular Endothelial Cells Under Benzo[a]pyrene Exposure.
    Article Snippet: .. Human microvascular endothelial cell line (HMEC-1) was sourced from the Center for Disease Control and Prevention, Atlanta, GA, USA; the cells were cultured in endothelial basal medium MCDB131 (US Biological Life Sciences, Ref E3000-01G, Salem, MA, USA) which contained 10% heat deactivated (56 ◦C, 30 min) fetal bovine serum (FBS, Dutscher, Ref 500105A1A, Bernolsheim, France), L-glutamine (Gibco, Ref 25030, 10 mM final, Paisley, Scotland, UK), penicillin/streptomycin (Gibco, Ref 15140-122, 100 unit/mL final), gentamycin (Gibco, Ref 15750-037, 500 μg/mL final), epithelial growth factor (EGF, Sigma, Ref E9644, 10 ng/mL final, Molsheim, France), and hydrocortisone (Up John, Ref 3400932141159, 1 μg/mL final, Paris, France) in 151.9 cm2 petri dishes (Corning, Ref 353025, Borre, France). .. The medium’s pH was adjusted to 7.6 using sodium bicarbonate (Gibco, Ref 25080-060) and replaced every 2–3 days.

    Article Title: Raman spectroscopy of large extracellular vesicles derived from human microvascular endothelial cells to detect benzo[a]pyrene exposure.
    Article Snippet: Extracellular vesicles (EVs) have shown great potential as biomarkers since they reflect the physio-pathological status of the producing cell.. In the context of cytotoxicity, it has been found that exposing cells to toxicants leads to changes in protein expression and the cargo of the EVs they produce.. Here, we studied large extracellular vesicles (lEVs) derived from human microvascular endothelial cells (HMEC-1) to detect the modifications induced by cell exposure to benzo[a]pyrene (B[a]P).

    Article Title: NanoBioAnalytical (NBA) Platform to Decipher Extracellular Vesicles Secreted by Microvascular Endothelial Cells Under Benzo[a]pyrene Exposure
    Article Snippet: .. Human microvascular endothelial cell line (HMEC-1) was sourced from the Center for Disease Control and Prevention, Atlanta, GA, USA; the cells were cultured in endothelial basal medium MCDB131 (US Biological Life Sciences, Ref E3000-01G, Salem, MA, USA) which contained 10% heat deactivated (56 °C, 30 min) fetal bovine serum (FBS, Dutscher, Ref 500105A1A, Bernolsheim, France), L-glutamine (Gibco, Ref 25030, 10 mM final, Paisley, Scotland, UK), penicillin/streptomycin (Gibco, Ref 15140-122, 100 unit/mL final), gentamycin (Gibco, Ref 15750-037, 500 μg/mL final), epithelial growth factor (EGF, Sigma, Ref E9644, 10 ng/mL final, Molsheim, France), and hydrocortisone (Up John, Ref 3400932141159, 1 μg/mL final, Paris, France) in 151.9 cm 2 petri dishes (Corning, Ref 353025, Borre, France). .. The medium’s pH was adjusted to 7.6 using sodium bicarbonate (Gibco, Ref 25080-060) and replaced every 2–3 days.



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